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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
04/09/2019 |
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Actualizado : |
16/03/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ROVIRA, P.J.; MCALLISTER, T.; LAKIN, S.M.; COOK, S.R.; DOSTER, E.; NOYES, N. R.; WEINROTH, M.D.; YANG, X.; PARKER, J. K.; BOUCHER, C.; BOOKER, C. W.; WOENER, D. R.; BELK, K. E.; MORLEY, P. S. |
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Afiliación : |
PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Department of Animal Sciences, College of Agricultural Sciences, Colorado State University, USA.; TIM MCALLISTER, Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada.; STEVEN M. LAKIN, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, USA.; SHAUN R. COOK, Alberta Agricultural and forestry, Lethbridge, Canada.; ENRIQUE DOSTER, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, USA.; NOELLE R. NOYES, Veterinary Population Medicine Department, University of Minnesota, USA.; MAGGIE D. WEINROTH, Department of Animal Sciences, College of Agricultural Sciences, Colorado State University, USA.; XIANG YANG, Department of Animal Science, University of California, Davis, USA.; JENNIFER K. PARKER, Deparment of Molecular Biosciences, University of Florida, Gainesville, FL, USA.; CHRISTINA BOUCHER, Deparment of Computer and Information Science and Engineering, University of Florida, Gainessville, FL, USA.; CALVIN W. BOOKER, Feedlot Health Management Services, Okotoks, AB, Canada.; DALE R. WOEMER, Deparment of Animal and Food Sciences, College of Agricultural Sciences & Natural Resources, Texas University, TX, USA.; KEITH E. BELK, Department of Animal Sciences, College of Agricultural Sciences, Colorado State University, USA.; PAUL S. MORLEY, VERO, Veterinary Education, Research , and Outreach Program, Texas A&M University and West Texas A&M University, Canyon, TX, USA. |
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Título : |
Characterization of the microbial resistome in conventional and "raised without antibiotics" beef and dairy production systems. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Frontier in Microbiology, September 2019. v. 10, article 1980, 11 p. OPEN ACCESS. |
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DOI : |
10.3389/fmicb.2019.01980 |
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Idioma : |
Inglés |
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Notas : |
Received 18 March 2019 // Accepted 12 August 2019 // Published 4 September 2019. |
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Contenido : |
Metagenomic investigations have the potential to provide unprecedented insights into microbial ecologies, such as those relating to antimicrobial resistance (AMR). We characterized the microbial resistome in livestock operations raising cattle conventionally (CONV) or without antibiotic exposures (RWA) using shotgun metagenomics. Samples of feces, wastewater from catchment basins, and soil where wastewater was applied were collected from CONV and RWA feedlot and dairy farms. After DNA extraction and sequencing, shotgun metagenomic reads were aligned to reference databases for identification of bacteria (Kraken) and antibiotic resistance genes (ARGs) accessions (MEGARes). Differences in microbial resistomes were found across farms with different production practices (CONV vs. RWA), types of cattle (beef vs. dairy), and types of sample (feces vs. wastewater vs. soil). Feces had the greatest number of ARGs per sample (mean = 118 and 79 in CONV and RWA, respectively), with tetracycline efflux pumps, macrolide phosphotransferases, and aminoglycoside nucleotidyltransferases mechanisms of resistance more abundant in CONV than in RWA feces. Tetracycline and macrolide-lincosamide-streptogramin classes of resistance were more abundant in feedlot cattle than in dairy cow feces, whereas the b-lactam class was more abundant in dairy cow feces. Lack of congruence between ARGs and microbial communities (procrustes analysis) suggested that other factors (e.g., location of farms, cattle source, management practices, diet, horizontal ARGs transfer, and co-selection of resistance), in addition to antimicrobial use, could have impacted resistome profiles. For that reason, we could not establish a cause-effect relationship between antimicrobial use and AMR, although ARGs in feces and effluents were associated with drug classes used to treat animals according to farms' records (tetracyclines and macrolides in feedlots, b-lactams in dairies), whereas ARGs in soil were dominated by multidrug resistance.
Characterization of the "resistance potential" of animal-derived and environmental samples is the first step toward incorporating metagenomic approaches into AMR surveillance in agricultural systems. Further research is needed to assess the publichealth risk associated with different microbial resistomes. MenosMetagenomic investigations have the potential to provide unprecedented insights into microbial ecologies, such as those relating to antimicrobial resistance (AMR). We characterized the microbial resistome in livestock operations raising cattle conventionally (CONV) or without antibiotic exposures (RWA) using shotgun metagenomics. Samples of feces, wastewater from catchment basins, and soil where wastewater was applied were collected from CONV and RWA feedlot and dairy farms. After DNA extraction and sequencing, shotgun metagenomic reads were aligned to reference databases for identification of bacteria (Kraken) and antibiotic resistance genes (ARGs) accessions (MEGARes). Differences in microbial resistomes were found across farms with different production practices (CONV vs. RWA), types of cattle (beef vs. dairy), and types of sample (feces vs. wastewater vs. soil). Feces had the greatest number of ARGs per sample (mean = 118 and 79 in CONV and RWA, respectively), with tetracycline efflux pumps, macrolide phosphotransferases, and aminoglycoside nucleotidyltransferases mechanisms of resistance more abundant in CONV than in RWA feces. Tetracycline and macrolide-lincosamide-streptogramin classes of resistance were more abundant in feedlot cattle than in dairy cow feces, whereas the b-lactam class was more abundant in dairy cow feces. Lack of congruence between ARGs and microbial communities (procrustes analysis) suggested that other factors (e.g., location of farms, cattle sour... Presentar Todo |
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Palabras claves : |
ANTIBIOTIC RESISTANCE; CATTLE; CATTLE BEEF; DAIRY CATTLE; METAGENOMICA; METAGENOMICS; MICROBIOMA; MICROBIOME; RESISTENCIA A ANTIBIÓTICOS; RESISTOME. |
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Thesagro : |
BOVINOS; BOVINOS DE CARNE; GANADO LECHERO. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13237/1/Rovira-Front-microb-2019.pdf
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Marc : |
LEADER 03681naa a2200457 a 4500
001 1060137
005 2021-03-16
008 2019 bl uuuu u00u1 u #d
024 7 $a10.3389/fmicb.2019.01980$2DOI
100 1 $aROVIRA, P.J.
245 $aCharacterization of the microbial resistome in conventional and "raised without antibiotics" beef and dairy production systems.$h[electronic resource]
260 $c2019
500 $aReceived 18 March 2019 // Accepted 12 August 2019 // Published 4 September 2019.
520 $aMetagenomic investigations have the potential to provide unprecedented insights into microbial ecologies, such as those relating to antimicrobial resistance (AMR). We characterized the microbial resistome in livestock operations raising cattle conventionally (CONV) or without antibiotic exposures (RWA) using shotgun metagenomics. Samples of feces, wastewater from catchment basins, and soil where wastewater was applied were collected from CONV and RWA feedlot and dairy farms. After DNA extraction and sequencing, shotgun metagenomic reads were aligned to reference databases for identification of bacteria (Kraken) and antibiotic resistance genes (ARGs) accessions (MEGARes). Differences in microbial resistomes were found across farms with different production practices (CONV vs. RWA), types of cattle (beef vs. dairy), and types of sample (feces vs. wastewater vs. soil). Feces had the greatest number of ARGs per sample (mean = 118 and 79 in CONV and RWA, respectively), with tetracycline efflux pumps, macrolide phosphotransferases, and aminoglycoside nucleotidyltransferases mechanisms of resistance more abundant in CONV than in RWA feces. Tetracycline and macrolide-lincosamide-streptogramin classes of resistance were more abundant in feedlot cattle than in dairy cow feces, whereas the b-lactam class was more abundant in dairy cow feces. Lack of congruence between ARGs and microbial communities (procrustes analysis) suggested that other factors (e.g., location of farms, cattle source, management practices, diet, horizontal ARGs transfer, and co-selection of resistance), in addition to antimicrobial use, could have impacted resistome profiles. For that reason, we could not establish a cause-effect relationship between antimicrobial use and AMR, although ARGs in feces and effluents were associated with drug classes used to treat animals according to farms' records (tetracyclines and macrolides in feedlots, b-lactams in dairies), whereas ARGs in soil were dominated by multidrug resistance. Characterization of the "resistance potential" of animal-derived and environmental samples is the first step toward incorporating metagenomic approaches into AMR surveillance in agricultural systems. Further research is needed to assess the publichealth risk associated with different microbial resistomes.
650 $aBOVINOS
650 $aBOVINOS DE CARNE
650 $aGANADO LECHERO
653 $aANTIBIOTIC RESISTANCE
653 $aCATTLE
653 $aCATTLE BEEF
653 $aDAIRY CATTLE
653 $aMETAGENOMICA
653 $aMETAGENOMICS
653 $aMICROBIOMA
653 $aMICROBIOME
653 $aRESISTENCIA A ANTIBIÓTICOS
653 $aRESISTOME
700 1 $aMCALLISTER, T.
700 1 $aLAKIN, S.M.
700 1 $aCOOK, S.R.
700 1 $aDOSTER, E.
700 1 $aNOYES, N. R.
700 1 $aWEINROTH, M.D.
700 1 $aYANG, X.
700 1 $aPARKER, J. K.
700 1 $aBOUCHER, C.
700 1 $aBOOKER, C. W.
700 1 $aWOENER, D. R.
700 1 $aBELK, K. E.
700 1 $aMORLEY, P. S.
773 $tFrontier in Microbiology, September 2019.$gv. 10, article 1980, 11 p. OPEN ACCESS.
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Registro original : |
INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
26/01/2021 |
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Actualizado : |
27/01/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BONIFACINO, S.; RESQUÍN, F.; LOPRETTI, M.; BUXEDAS, L.; VÁZQUEZ, S.; GONZÁLEZ, M.; SAPOLINSKI, A.; HIRIGOYEN, A.; DOLDÁN, J.; RACHID, C.; CARRASCO-LETELIER, L. |
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Afiliación : |
SILVANA BONIFACINO, Laboratorio de Técnicas nucleares aplicadas en Bioquímica y Biotecnología, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; JOSE FERNANDO RESQUIN PEREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARY LOPRETTI, Laboratorio de Técnicas nucleares aplicadas en Bioquímica y Biotecnología, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; I&D Biotecnología, Laboratorio Tecnol#19;ogico de Uruguay (LATU); LUCIANA BUXEDAS, Laboratorio de Técnicas nucleares aplicadas en Bioquímica y Biotecnología, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; SYLVIA VÁZQUEZ, Laboratorio de Técnicas nucleares aplicadas en Bioquímica y Biotecnología, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; MARIANA GONZÁLEZ, I&D Biotecnología, Laboratorio Tecnologico de Uruguay (LATU); ALEJANDRA SAPOLINSKI, I&D Biotecnología, Laboratorio Tecnologico de Uruguay (LATU); ANDRES EDUARDO HIRIGOYEN DOMINGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JAVIER DOLDÁN, Departamento de Forestales, Laboratorio Tecnol#19;ogico de Uruguay (LATU), Montevideo, Uruguay; ANA CECILIA RACHID CASNATI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LEONIDAS CARRASCO-LETELIER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Bioethanol production using high density Eucalyptus crops in Uruguay [Research article] |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Heliyon, January 2021, Volume 7, Issue 1, e06031. OPEN ACCESS. Doi: https://doi.org/10.1016/j.heliyon.2021.e06031 |
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DOI : |
10.1016/j.heliyon.2021.e06031 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 9 August 2020; Received in revised form 16 November 2020; Accepted 14 January 2021. Corresponding author. E-mail address: silvana.bonifacino@gmail.com (S. Bonifacino). Acknowledgements: The authors thank the Forestal Oriental Company for its collaborationin thefield experiments and planting. |
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Contenido : |
Experimental scale crops for Eucalyptus grandis, Eucalyptus benthamii, Eucalyptus dunnii and Eucalyptus tereticornis, at 2,220, 4,440 and 6,660 trees ha?1 were established in two soil units, at Paysandú and Tacuarembó, Uruguay. Wood samples were taken from twenty-two-months-old trees, and were used to produce bioethanol by pre-hydrolysis simultaneous saccharyfication and fermentation process (PSSF). Cellulose and lignin content was analyzed. Species and planting density affected biomass production at both sites; the highest value was obtained with E. dunnii at 6,660 trees ha?1 at Paysandú. Cellulose content of wood varied between species at both sites, but only between planting densities at Tacuarembó. The site effect showed that the highest amount of cellulose (14.7 Mg ha?1) was produced at Paysandú. E. benthamii and E. tereticornis wood showed higher lignin contents, conversely, the PSSF yields showed no differences, which led to a bioethanol average of 97 L Mg?1. Bioethanol productivity was associated to the biomass productivity. It was possible to obtain 2,650 L ha?1 of bioethanol using wood from E. benthamii, E. dunnii and E. grandis at 4,440 and 6,660 trees ha?1 at Paysandú, and with E. benthamii at 4,440 and 6,660 trees ha?1, and E. dunnii at 6,660 trees ha?1 at Tacuarembó. |
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Palabras claves : |
BIOETHANOL; BIOMASS; EUCALYPTUS SPECIES; HIGH PLANTING DENSITY; PRODUCCIÓN FORESTAL; SHORT ROTATION FORESTRY. |
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Asunto categoría : |
K10 Producción forestal |
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URL : |
https://www.sciencedirect.com/sdfe/reader/pii/S2405844021001365/pdf
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Marc : |
LEADER 02643naa a2200337 a 4500
001 1061681
005 2021-01-27
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1016/j.heliyon.2021.e06031$2DOI
100 1 $aBONIFACINO, S.
245 $aBioethanol production using high density Eucalyptus crops in Uruguay [Research article]$h[electronic resource]
260 $c2021
500 $aArticle history: Received 9 August 2020; Received in revised form 16 November 2020; Accepted 14 January 2021. Corresponding author. E-mail address: silvana.bonifacino@gmail.com (S. Bonifacino). Acknowledgements: The authors thank the Forestal Oriental Company for its collaborationin thefield experiments and planting.
520 $aExperimental scale crops for Eucalyptus grandis, Eucalyptus benthamii, Eucalyptus dunnii and Eucalyptus tereticornis, at 2,220, 4,440 and 6,660 trees ha?1 were established in two soil units, at Paysandú and Tacuarembó, Uruguay. Wood samples were taken from twenty-two-months-old trees, and were used to produce bioethanol by pre-hydrolysis simultaneous saccharyfication and fermentation process (PSSF). Cellulose and lignin content was analyzed. Species and planting density affected biomass production at both sites; the highest value was obtained with E. dunnii at 6,660 trees ha?1 at Paysandú. Cellulose content of wood varied between species at both sites, but only between planting densities at Tacuarembó. The site effect showed that the highest amount of cellulose (14.7 Mg ha?1) was produced at Paysandú. E. benthamii and E. tereticornis wood showed higher lignin contents, conversely, the PSSF yields showed no differences, which led to a bioethanol average of 97 L Mg?1. Bioethanol productivity was associated to the biomass productivity. It was possible to obtain 2,650 L ha?1 of bioethanol using wood from E. benthamii, E. dunnii and E. grandis at 4,440 and 6,660 trees ha?1 at Paysandú, and with E. benthamii at 4,440 and 6,660 trees ha?1, and E. dunnii at 6,660 trees ha?1 at Tacuarembó.
653 $aBIOETHANOL
653 $aBIOMASS
653 $aEUCALYPTUS SPECIES
653 $aHIGH PLANTING DENSITY
653 $aPRODUCCIÓN FORESTAL
653 $aSHORT ROTATION FORESTRY
700 1 $aRESQUÍN, F.
700 1 $aLOPRETTI, M.
700 1 $aBUXEDAS, L.
700 1 $aVÁZQUEZ, S.
700 1 $aGONZÁLEZ, M.
700 1 $aSAPOLINSKI, A.
700 1 $aHIRIGOYEN, A.
700 1 $aDOLDÁN, J.
700 1 $aRACHID, C.
700 1 $aCARRASCO-LETELIER, L.
773 $tHeliyon, January 2021, Volume 7, Issue 1, e06031. OPEN ACCESS. Doi: https://doi.org/10.1016/j.heliyon.2021.e06031
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